Swiprosin-2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB65980-50, MB65980-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9BUP0

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB (1/1000 - 1/2000), IF/ICC (1/100 - 1/200)

Background: Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899).

May play a role in neuronal differentiation (By similarity).

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Swiprosin-2 protein.

Bioworld Molecular Weight: ~ 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Swiprosin-2 expression in mouse spleen (A), rat spleen (B) whole cell lysates., Immunofluorescent analysis of Swiprosin-2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: SWS2; EF-hand domain-containing protein D1; EF-hand domain-containing protein 1; Swiprosin-2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Swiprosin-2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified